Since the single cell sequencing technology came out in 2009 and was named the annual technology by Nature Methods in 2013, it has been increasingly used in the field of scientific research.
Recently, we have focused on a company in the field of single cell sequencing-Beijing Geek Gene Technology Co., Ltd. (hereinafter referred to as “Geek Gene”), which was established in 2016 with a registered capital of 2.0404816 million. The most recent shareholding change occurred on November 28, 2019, and received a capital increase from Haidian District, a state-owned joint venture of Haidian District, a subsidiary of the Haidian Park Entrepreneurship Service Center of Zhongguancun Science Park. Set up branches in both places.
Geek Gene is committed to developing a new generation of personalized and precise drug delivery and drug discovery for cancer patients based on a small number of cell sequencing technologies and primary tumor organ body culture platforms. Focus on single cell and small cell sequencing technology research and development, services and applications, such as single cell whole genome sequencing, single cell transcriptome sequencing, single cell epigenetics research, etc.
According to the information on the official website of Lilac Tong and Geek Gene, the main service items of Geek Gene are as follows:
1. Low-cost sequencing of single-cell transcriptomes (sc RNAseq (barcoeded)): Mining large numbers of genes between single-cell samples simultaneously in a high-throughput and low-cost manner throughout the genome Differential expression is suitable for systems with highly heterogeneous tumor cells, early embryonic cell populations, and stem cell differentiation.
2. Single-cell transcriptome sequencing (sc RNAseq (smart-seq2)): The improved smart-seq2 technology can meet the high sensitivity and non-preferred cDNA amplification, which is different in analysis In addition to the differential expression between cells, information such as fusion genes, variable splicing, and gene mutations in cells can also be deeply detected.
3. Single cell restricted representative region methylation sequencing (sc RRBS): A large portion of CpG sites with important information, including 70 More than% of promoter regions and more than 80% of methylated islands.
4. Single-cell genome-wide methylation sequencing (sc WGBS): Through optimization of the single-cell genome-wide methylation step, it can cover the whole genome CpG methylation more comprehensively Location to more than 50%.
5. Single-cell whole-genome c-sequencing (sc WGS): The improved MALBAC technology is used, which has the characteristics of low bias and more uniform amplification, so it can accurately determine the fineWhether the cell has copy number variation.
6. Single cell whole exome sequencing (sc WES): The optimized MDA technology is used to first amplify the single cell genome, which has the characteristics of low error rate and higher coverage , Exon sequencing of amplified products can more accurately obtain single-base mutations in single-cell exons.
Geek gene product price
On November 28, 2019, Geek Gene received a capital increase from Beijing Golden Seed Technology Innovation Center, which belongs to Haichuang Elite, with an amount of 41,000, accounting for 2% of the shares. Haichuang Elite is the entrepreneurial service center of Haidian Park in Zhongguancun Science and Technology Park A subsidiary of Haidian District, a state-owned company.
Beijing Geek Gene Technology Co., Ltd. shareholding structure
Public information shows that Bao Renyan, one of the company’s principals, is a Ph.D. in biology from Beijing Forestry University. He is currently the Geek Gene Technical Support Director and was engaged in biomedical industry research at Beijing Academy of Science and Technology. In-depth research on the development of genetic technology industry and related frontier technologies.
In addition, Geek Gene has two other foreign investment companies. They are Guangdong Foshan Geek Gene Technology Co., Ltd. and Wuxi Jiyin Technology Co., Ltd. Among them, 3 million yuan was invested in Foshan Geek Gene, accounting for 20% of the shares, Bao Renyan personally invested 4.95 million yuan, accounting for 33% of the shares; Wuxi Ji invested 1 million yuan, accounting for 100% of the shares, and was its wholly-owned subsidiary.